An Active Type I-E CRISPR-Cas System Identified in Streptomyces avermitilis
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چکیده
منابع مشابه
Detection and characterization of spacer integration intermediates in type I-E CRISPR–Cas system
The adaptation against foreign nucleic acids by the CRISPR-Cas system (Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins) depends on the insertion of foreign nucleic acid-derived sequences into the CRISPR array as novel spacers by still unknown mechanism. We identified and characterized in Escherichia coli intermediate states of spacer integration and mapp...
متن کاملThe type I-E CRISPR-Cas system Biology and applications of an adaptive immune system in bacteria
Amlinger, L. 2017. The type I-E CRISPR-Cas system. Biology and applications of an adaptive immune system in bacteria. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1466. 61 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9787-3. CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered reg...
متن کاملDisabling a Type I-E CRISPR-Cas Nuclease with a Bacteriophage-Encoded Anti-CRISPR Protein
CRISPR (clustered regularly interspaced short palindromic repeat)-Cas adaptive immune systems are prevalent defense mechanisms in bacteria and archaea. They provide sequence-specific detection and neutralization of foreign nucleic acids such as bacteriophages and plasmids. One mechanism by which phages and other mobile genetic elements are able to overcome the CRISPR-Cas system is through the e...
متن کاملA New Group of Phage Anti-CRISPR Genes Inhibits the Type I-E CRISPR-Cas System of Pseudomonas aeruginosa
CRISPR-Cas systems are one of the most widespread phage resistance mechanisms in prokaryotes. Our lab recently identified the first examples of phage-borne anti-CRISPR genes that encode protein inhibitors of the type I-F CRISPR-Cas system of Pseudomonas aeruginosa. A key question arising from this work was whether there are other types of anti-CRISPR genes. In the current work, we address this ...
متن کاملEasy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
BACKGROUND Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compared with the widely used type II system, which expressed dCas9 as an additional plasmid. RESULTS...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0149533